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prl-null internal renilla luciferase control construct  (Promega)

 
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    Structured Review

    Promega prl-null internal renilla luciferase control construct
    Prl Null Internal Renilla Luciferase Control Construct, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/prl-null+internal+renilla+luciferase+control+construct/pgl3+basic/10__1128_slash_mbio__02267___21-222-25-31
    Average 90 stars, based on 1 article reviews
    prl-null internal renilla luciferase control construct - by Bioz Stars, 2026-10
    90/100 stars

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    Related Articles

    Luciferase:

    Article Title: Human Cytomegalovirus UL138 Protein Inhibits the STING Pathway and Reduces Interferon Beta mRNA Accumulation during Lytic and Latent Infections
    Article Snippet: .. For luciferase reporter assays, cells were cotransfected with an IFN-b promoter-driven firefly luciferase construct (a gift from Blossom Damania, UNC—Chapel Hill) along with pRL-tK or pRL-null internal Renilla luciferase control construct (Promega) and expression constructs for the desired proteins or a matched empty vector control. .. Forty-eight hours posttransfection, cells were washed with phosphatebuffered saline (PBS; Invitrogen) and lysed in 1 passive lysis buffer (PLB; Promega).

    Construct:

    Article Title: Human Cytomegalovirus UL138 Protein Inhibits the STING Pathway and Reduces Interferon Beta mRNA Accumulation during Lytic and Latent Infections
    Article Snippet: .. For luciferase reporter assays, cells were cotransfected with an IFN-b promoter-driven firefly luciferase construct (a gift from Blossom Damania, UNC—Chapel Hill) along with pRL-tK or pRL-null internal Renilla luciferase control construct (Promega) and expression constructs for the desired proteins or a matched empty vector control. .. Forty-eight hours posttransfection, cells were washed with phosphatebuffered saline (PBS; Invitrogen) and lysed in 1 passive lysis buffer (PLB; Promega).

    Control:

    Article Title: Human Cytomegalovirus UL138 Protein Inhibits the STING Pathway and Reduces Interferon Beta mRNA Accumulation during Lytic and Latent Infections
    Article Snippet: .. For luciferase reporter assays, cells were cotransfected with an IFN-b promoter-driven firefly luciferase construct (a gift from Blossom Damania, UNC—Chapel Hill) along with pRL-tK or pRL-null internal Renilla luciferase control construct (Promega) and expression constructs for the desired proteins or a matched empty vector control. .. Forty-eight hours posttransfection, cells were washed with phosphatebuffered saline (PBS; Invitrogen) and lysed in 1 passive lysis buffer (PLB; Promega).

    Expressing:

    Article Title: Human Cytomegalovirus UL138 Protein Inhibits the STING Pathway and Reduces Interferon Beta mRNA Accumulation during Lytic and Latent Infections
    Article Snippet: .. For luciferase reporter assays, cells were cotransfected with an IFN-b promoter-driven firefly luciferase construct (a gift from Blossom Damania, UNC—Chapel Hill) along with pRL-tK or pRL-null internal Renilla luciferase control construct (Promega) and expression constructs for the desired proteins or a matched empty vector control. .. Forty-eight hours posttransfection, cells were washed with phosphatebuffered saline (PBS; Invitrogen) and lysed in 1 passive lysis buffer (PLB; Promega).

    Plasmid Preparation:

    Article Title: Human Cytomegalovirus UL138 Protein Inhibits the STING Pathway and Reduces Interferon Beta mRNA Accumulation during Lytic and Latent Infections
    Article Snippet: .. For luciferase reporter assays, cells were cotransfected with an IFN-b promoter-driven firefly luciferase construct (a gift from Blossom Damania, UNC—Chapel Hill) along with pRL-tK or pRL-null internal Renilla luciferase control construct (Promega) and expression constructs for the desired proteins or a matched empty vector control. .. Forty-eight hours posttransfection, cells were washed with phosphatebuffered saline (PBS; Invitrogen) and lysed in 1 passive lysis buffer (PLB; Promega).



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